TY - JOUR
T1 - The transcriptome enables the identification of candidate genes behind medicinal value of Drumstick tree (Moringa oleifera)
AU - Pasha, Shaik Naseer
AU - Shafi, K. Mohamed
AU - Joshi, Adwait G.
AU - Meenakshi, Iyer
AU - Harini, K.
AU - Mahita, Jarjapu
AU - Sajeevan, Radha Sivarajan
AU - Karpe, Snehal D.
AU - Ghosh, Pritha
AU - Nitish, Sathyanarayanan
AU - Gandhimathi, A.
AU - Mathew, Oommen K.
AU - Prasanna, Subramanian Hari
AU - Malini, Manoharan
AU - Mutt, Eshita
AU - Naika, Mahantesha
AU - Ravooru, Nithin
AU - Rao, Rajas M.
AU - Shingate, Prashant N.
AU - Sukhwal, Anshul
AU - Sunitha, Margaret S.
AU - Upadhyay, Atul K.
AU - Vinekar, Rithvik S.
AU - Sowdhamini, Ramanathan
PY - 2020
Y1 - 2020
N2 - Moringa oleifera is a plant well-known for its nutrition value, drought resistance and medicinal properties. cDNA libraries from five different tissues (leaf, root, stem, seed and flower) of M. oleifera cultivar Bhagya were generated and sequenced. We developed a bioinformatics pipeline to assemble transcriptome, along with the previously published M. oleifera genome, to predict 17,148 gene models. Few candidate genes related to biosynthesis of secondary metabolites, vitamins and ion transporters were identified. Expressions were further confirmed by real-time quantitative PCR experiments for few promising leads. Quantitative estimation of metabolites, as well as elemental analysis, was also carried out to support our observations. Enzymes in the biosynthesis of vitamins and metabolites like quercetin and kaempferol are highly expressed in leaves, flowers and seeds. The expression of iron transporters and calcium storage proteins were observed in root and leaves. In general, leaves retain the highest amount of small molecules of interest.
AB - Moringa oleifera is a plant well-known for its nutrition value, drought resistance and medicinal properties. cDNA libraries from five different tissues (leaf, root, stem, seed and flower) of M. oleifera cultivar Bhagya were generated and sequenced. We developed a bioinformatics pipeline to assemble transcriptome, along with the previously published M. oleifera genome, to predict 17,148 gene models. Few candidate genes related to biosynthesis of secondary metabolites, vitamins and ion transporters were identified. Expressions were further confirmed by real-time quantitative PCR experiments for few promising leads. Quantitative estimation of metabolites, as well as elemental analysis, was also carried out to support our observations. Enzymes in the biosynthesis of vitamins and metabolites like quercetin and kaempferol are highly expressed in leaves, flowers and seeds. The expression of iron transporters and calcium storage proteins were observed in root and leaves. In general, leaves retain the highest amount of small molecules of interest.
KW - Plant transcriptome
KW - Functional annotation
KW - Differential expression
KW - Metabolic pathway analysis
KW - Plant transcriptome
KW - Functional annotation
KW - Differential expression
KW - Metabolic pathway analysis
UR - https://res.slu.se/id/publ/120211
U2 - 10.1016/j.ygeno.2019.04.014
DO - 10.1016/j.ygeno.2019.04.014
M3 - Journal article
SN - 0888-7543
VL - 112
SP - 621
EP - 628
JO - Genomics
JF - Genomics
IS - 1
ER -