Sustainable biochemicals - Extraction of lipids and carotenoids from the oleaginous yeast Rhodotorula toruloides

Yashaswini Nagavara Nagaraj

doi:10.54612/a.32l4mguu85

Sustainable biochemicals - Extraction of lipids and carotenoids from the oleaginous yeast Rhodotorula toruloides

Bidragets översatta titel: Uthålliga biokemikalier-extraktion av lipider och karotenoider från Rhodotorula toruloides, en oleaginös jäst

Yashaswini Nagavara Nagaraj

Institutionen för molekylära vetenskaper

Publikation: Avhandling › Doktorsavhandling

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Sammanfattning

Oleaginösa jästsvampar med sin stora förmåga att lagra lipider ger oss hållbara alternativ till vegetabiliska oljor. Dessa mikroorganismer kan också syntetisera karotenoider tillsammans med lipider. De har förmågan att trivas på olika avfallsoch lågkostnadskolhydratkällor. I denna avhandling har vi undersökt olika sätt att omvandla lignocellulosa biomassa till fettsyror och karotenoider med hjälp av oleaginösa jästsvampar och potentiella nya metoder för att effektivt extrahera dessa värdefulla föreningar, med det övergripande målet att etablera en plattform för deras framtida användning som viktiga ingredienser inom livsmedels- och foderindustrin.Omfattande undersökningar av konventionella och superkritisk koldioxid (SC-CO2) extraktionsmetoders påverkan på sammansättningen av lipid- och karotenoidextrakt som härstammar från jästsvamparten Rhodotorula toruloides visar att SC-CO2 är en lovande extraktionsmetod för att isolera dessa föreningar, särskilt karotenoider, till skillnad från konventionella extraktionsmetoder. Inverkan av förtvålning i konventionella extraktionsmetoder på utbyte av enskilda karotenoider upptäcktes. Ett behov att ompröva och förbättra befintliga metoder för extraktion av lipider och karotenoider, finns. Analys av lipid- och karotenoidprofiler hos olika R. toruloidesstammar gav värdefulla insikter, särskilt i hybridstammen CBS 6016T , för vilken en övervägande ärftlig och uttrycksrelaterad genetiska egenskaper från en föräldrastam (CBS 14), men inte den andra (CBS 349). Dessa forskningsresultat har en betydande bioteknologisk potential. SC-CO2-extraktion ökar möjligheten till miljövänlig produktion av lipider och karotenoider från R. toruloides, medan kunskapen om hybridstammen CBS 6016T kan hjälpa till med genetiskt ingenjörskap för att uppnå högre avkastning. Sammanfattningsvis ger resultaten i denna avhandling grönare och mer effektiva processer inom livsmedel, foder och bioteknik.

Bidragets översatta titel	Uthålliga biokemikalier-extraktion av lipider och karotenoider från Rhodotorula toruloides, en oleaginös jäst
Originalspråk	Engelska
Kvalifikation	Doktor i filosofi
Förlag	Swedish University of Agricultural Sciences
Tryckta ISBN	978-91-8046-218-1
Elektroniska ISBN	978-91-8046-219-8
DOI	https://doi.org/10.54612/a.32l4mguu85
Status	Publicerad - 2023

Nyckelord

R. toruloides strains
Rhodotorula toruloides
carotenoids
lignocellulose
lipid
saponification
supercritical carbon dioxide extraction

SLU-serie

Acta Universitatis Agriculturae Sueciae

Åtkomst av dokument

10.54612/a.32l4mguu85

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title = "Sustainable biochemicals - Extraction of lipids and carotenoids from the oleaginous yeast Rhodotorula toruloides",

abstract = "Oleaginous yeasts, with their high lipid accumulation, offer potential as sustainable alternatives to vegetable oils. They also have a unique capability for accumulating carotenoids together with lipids. Also, they have the ability to thrive on various waste and low-cost carbon sources. This thesis examined ways of converting lignocellulosic biomass into fatty acids and carotenoids using oleaginous yeasts and potential methods for extracting these valuable compounds efficiently, with the ultimate aim of establishing a foundation for their future incorporation as essential ingredients in the food and feed industries.Comprehensive investigations into the impact of conventional and supercritical carbon dioxide (SC-CO2) extraction methods on the composition of lipid and carotenoid extracts derived from the yeast species Rhodotorula toruloides revealed that SC-CO2 has promising capability for isolating these compounds, particularly carotenoids, unlike conventional extraction methods. A previously undetected adverse influence of including saponification in conventional methods on yield and composition of carotenoids was observed. This novel insight highlights the need for re-evaluating and refining existing lipid and carotenoid extraction methods. Further analysis of the lipid and carotenoid profiles of various R. toruloides strains yielded valuable insights, particularly concerning the hybrid strain CBS 6016T , for which predominant inheritance and expression of genetic traits from one parental strain (CBS 14), but not the other (CBS 349), was revealed. This research offers significant biotechnological potential. SC-CO2 extraction enhances eco-friendly lipid and carotenoid production in R. toruloides, while insights into hybrid strain CBS 6016T can aid genetic engineering for higher yields. Overall, this thesis opens the way toward greener, more efficient processes in the food, feed, and biotech sectors.",

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N2 - Oleaginous yeasts, with their high lipid accumulation, offer potential as sustainable alternatives to vegetable oils. They also have a unique capability for accumulating carotenoids together with lipids. Also, they have the ability to thrive on various waste and low-cost carbon sources. This thesis examined ways of converting lignocellulosic biomass into fatty acids and carotenoids using oleaginous yeasts and potential methods for extracting these valuable compounds efficiently, with the ultimate aim of establishing a foundation for their future incorporation as essential ingredients in the food and feed industries.Comprehensive investigations into the impact of conventional and supercritical carbon dioxide (SC-CO2) extraction methods on the composition of lipid and carotenoid extracts derived from the yeast species Rhodotorula toruloides revealed that SC-CO2 has promising capability for isolating these compounds, particularly carotenoids, unlike conventional extraction methods. A previously undetected adverse influence of including saponification in conventional methods on yield and composition of carotenoids was observed. This novel insight highlights the need for re-evaluating and refining existing lipid and carotenoid extraction methods. Further analysis of the lipid and carotenoid profiles of various R. toruloides strains yielded valuable insights, particularly concerning the hybrid strain CBS 6016T , for which predominant inheritance and expression of genetic traits from one parental strain (CBS 14), but not the other (CBS 349), was revealed. This research offers significant biotechnological potential. SC-CO2 extraction enhances eco-friendly lipid and carotenoid production in R. toruloides, while insights into hybrid strain CBS 6016T can aid genetic engineering for higher yields. Overall, this thesis opens the way toward greener, more efficient processes in the food, feed, and biotech sectors.

AB - Oleaginous yeasts, with their high lipid accumulation, offer potential as sustainable alternatives to vegetable oils. They also have a unique capability for accumulating carotenoids together with lipids. Also, they have the ability to thrive on various waste and low-cost carbon sources. This thesis examined ways of converting lignocellulosic biomass into fatty acids and carotenoids using oleaginous yeasts and potential methods for extracting these valuable compounds efficiently, with the ultimate aim of establishing a foundation for their future incorporation as essential ingredients in the food and feed industries.Comprehensive investigations into the impact of conventional and supercritical carbon dioxide (SC-CO2) extraction methods on the composition of lipid and carotenoid extracts derived from the yeast species Rhodotorula toruloides revealed that SC-CO2 has promising capability for isolating these compounds, particularly carotenoids, unlike conventional extraction methods. A previously undetected adverse influence of including saponification in conventional methods on yield and composition of carotenoids was observed. This novel insight highlights the need for re-evaluating and refining existing lipid and carotenoid extraction methods. Further analysis of the lipid and carotenoid profiles of various R. toruloides strains yielded valuable insights, particularly concerning the hybrid strain CBS 6016T , for which predominant inheritance and expression of genetic traits from one parental strain (CBS 14), but not the other (CBS 349), was revealed. This research offers significant biotechnological potential. SC-CO2 extraction enhances eco-friendly lipid and carotenoid production in R. toruloides, while insights into hybrid strain CBS 6016T can aid genetic engineering for higher yields. Overall, this thesis opens the way toward greener, more efficient processes in the food, feed, and biotech sectors.

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KW - supercritical carbon dioxide extraction

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