Metabolomic and proteomic changes in Standardbred horses in training

Lisa Johansson

doi:10.54612/a.nndknetvqo

Metabolomic and proteomic changes in Standardbred horses in training

Lisa Johansson

Publikation: Avhandling › Doktorsavhandling

6 Nedladdningar

Sammanfattning

I min avhandling tittade vi på metabolomik och proteomik förändringar i blodplasma-prover från 16 travhästar från 1.5 års ålder till 3.5 års ålder. Alla hästar hade samma träningsprogram från september när de var 1.5 år till mars när de var 2 år. I mars introducerades hög-intensiv träning och hästarna delades in i två olika träningsgrupper, Hög och Låg. Båda grupperna följde samma träningsprogram med skillnaden att träningsgruppen Låg hade 30% kortare hög-intensiv träningsdistans jämfört med träningsgrupp Hög. Båda grupperna tränades också i samma hastighet. Blodprover togs från 1,5 till 3,5 års ålder. Insulin-like growth factor 1 (IGF-1) analyserades med ett ELISA kit. Metabolomikprofilen analyserades med en riktad absolut kvantitativ masspektrometri och proteomiken analyserades med en oriktad masspektrometri. Inga signifikanta skillnader i IGF-1-koncentrationen hittades mellan träningsgrupperna men ett avbrott i den förväntade nedgången i IGF-1 observerades och den inträffade samtidigt som hög-intensiv träning introducerades. Skillnader i metabolomik-profilen mellan träningsgrupperna hittades bara vid 2 års ålder men skillnader över tid kunde hittas för alla åldrar (2, 2.5 och 3.5) jämfört med 1.5 års ålder. Metaboliterna som skilde signifikant för både träningsgrupper och över tid var associerade med aerob energiproduktion och aminosyrametabolismen, och kanske också med pH-buffring och kardiovaskulära förändringar. Proteomikanalysen visade inte på några signifikanta skillnader mellan träningsgrupperna men 17 proteiner skiljde sig signifikant över tid och de var relaterade till energimetabolism, bentillväxt och cirkulationsfunktioner. Sammanfattningsvis visade studierna att både metabolomik- och proteomik-profilen förändrades över tid, men att skillnader mellan träningsgrupperna, bara kunde observeras i metabolomik-profilen.

Originalspråk	Engelska
Kvalifikation	Doktor i filosofi
Förlag	Swedish University of Agricultural Sciences
Tryckta ISBN	978-91-8046-284-6
Elektroniska ISBN	978-91-8046-285-3
DOI	https://doi.org/10.54612/a.nndknetvqo
Status	Publicerad - 2024

Nyckelord

IGF-1
Metabolomics
equine
exercise
high-intensity training
proteomics

SLU-serie

Acta Universitatis Agriculturae Sueciae

Åtkomst av dokument

10.54612/a.nndknetvqo

FulltextSlutlig publicerad version, 2,05 MBLicens: CC BY-NC-ND

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title = "Metabolomic and proteomic changes in Standardbred horses in training",

abstract = "Metabolomic and proteomic changes in blood plasma were analysed in 16 young Standardbred horses from the age of 1.5 to 3.5 years. All horses had the same training programme from September as 1.5-year-olds until March as 2-year-olds, when highintensity training was introduced and the horses were divided into two training groups, High and Low. Both groups followed the same training programme, but the Low group performed 30\% shorter high-intensity training distances than the High group. The same speed was aimed for with both groups. In blood samples collected from age 1.5 to 3.5 years, insulin-like growth factor 1 (IGF-1) was analysed with an ELISA kit, metabolomic profile was analysed using targeted absolute quantitative mass spectrometry and proteomic profile was analysed using untargeted mass spectrometry. There was no significant difference in IGF-1 between the training groups, but the expected ongoing IGF-1 decline was interrupted at a time which coincided with the onset of high-intensity training. Metabolomic differences between the training groups were only observed at 2 years of age, but concentrations of several metabolites changed significantly over time compared with at 1.5 years of age. Metabolites that differed significantly between the training groups and over time are associated with aerobic energy production and amino acid metabolism, and potentially also pH-buffering and vascular responses. The proteomics data did not reveal any significant differences between the training groups but the concentrations of 17 proteins related to energy metabolism, bone formation and circulatory functions changed significantly over time. In summary, both the metabolomic and proteomic profile in young horses in training changed over time, while the metabolic profile was also affected by training programme.",

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N2 - Metabolomic and proteomic changes in blood plasma were analysed in 16 young Standardbred horses from the age of 1.5 to 3.5 years. All horses had the same training programme from September as 1.5-year-olds until March as 2-year-olds, when highintensity training was introduced and the horses were divided into two training groups, High and Low. Both groups followed the same training programme, but the Low group performed 30% shorter high-intensity training distances than the High group. The same speed was aimed for with both groups. In blood samples collected from age 1.5 to 3.5 years, insulin-like growth factor 1 (IGF-1) was analysed with an ELISA kit, metabolomic profile was analysed using targeted absolute quantitative mass spectrometry and proteomic profile was analysed using untargeted mass spectrometry. There was no significant difference in IGF-1 between the training groups, but the expected ongoing IGF-1 decline was interrupted at a time which coincided with the onset of high-intensity training. Metabolomic differences between the training groups were only observed at 2 years of age, but concentrations of several metabolites changed significantly over time compared with at 1.5 years of age. Metabolites that differed significantly between the training groups and over time are associated with aerobic energy production and amino acid metabolism, and potentially also pH-buffering and vascular responses. The proteomics data did not reveal any significant differences between the training groups but the concentrations of 17 proteins related to energy metabolism, bone formation and circulatory functions changed significantly over time. In summary, both the metabolomic and proteomic profile in young horses in training changed over time, while the metabolic profile was also affected by training programme.

AB - Metabolomic and proteomic changes in blood plasma were analysed in 16 young Standardbred horses from the age of 1.5 to 3.5 years. All horses had the same training programme from September as 1.5-year-olds until March as 2-year-olds, when highintensity training was introduced and the horses were divided into two training groups, High and Low. Both groups followed the same training programme, but the Low group performed 30% shorter high-intensity training distances than the High group. The same speed was aimed for with both groups. In blood samples collected from age 1.5 to 3.5 years, insulin-like growth factor 1 (IGF-1) was analysed with an ELISA kit, metabolomic profile was analysed using targeted absolute quantitative mass spectrometry and proteomic profile was analysed using untargeted mass spectrometry. There was no significant difference in IGF-1 between the training groups, but the expected ongoing IGF-1 decline was interrupted at a time which coincided with the onset of high-intensity training. Metabolomic differences between the training groups were only observed at 2 years of age, but concentrations of several metabolites changed significantly over time compared with at 1.5 years of age. Metabolites that differed significantly between the training groups and over time are associated with aerobic energy production and amino acid metabolism, and potentially also pH-buffering and vascular responses. The proteomics data did not reveal any significant differences between the training groups but the concentrations of 17 proteins related to energy metabolism, bone formation and circulatory functions changed significantly over time. In summary, both the metabolomic and proteomic profile in young horses in training changed over time, while the metabolic profile was also affected by training programme.

KW - IGF-1

KW - Metabolomics

KW - equine

KW - exercise

KW - high-intensity training

KW - proteomics

KW - IGF-1

KW - Metabolomics

KW - equine

KW - exercise

KW - high-intensity training

KW - proteomics

UR - https://res.slu.se/id/publ/127133

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M3 - Doctoral thesis

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PB - Swedish University of Agricultural Sciences

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