TY - JOUR
T1 - A single carbocyclic nucleotide substitution in a 12mer DNA gives a Hoogsteen basepaired duplex (till 38 degrees C) and a hairpin (till 65 degrees C). A 600 MHZ NMR spectroscopic study.
AU - Isaksson, J.
AU - Maltseva, T.
AU - Agback, Peter
AU - Luo, X.
AU - Kumar, A.
AU - Zamaratski, E.
AU - Chattopadhyaya, J.
PY - 1999
Y1 - 1999
N2 - The impact of intramolecular stereoelectronic effects has been examined by comparison of the solution structures of natural oligo-DNA duplex, 5'((1)C(2)G(3)C(4)G(5)A(6)A(7)T(8)T(9)C(10)G(11)C(12)G)(2)(3'), and its carbocyclic-nucleotide analogues in which the pentose sugar in 2'-dA residue is replaced with its carbocyclic counterpart (i.e. 2'-deoxyaristeromycin). Based on the NMR evidences, it has been shown, that 2'-deoxyaristeromycin analog exists in a dynamic equilibrium between the two forms of duplexes, one with W-C bp and the second with Hoogsteen bp in ca 1:1 ratio at lower temperature (below 35 degrees C) and as hairpin at higher temperature (from similar to 40 degrees - 60 degrees C).
AB - The impact of intramolecular stereoelectronic effects has been examined by comparison of the solution structures of natural oligo-DNA duplex, 5'((1)C(2)G(3)C(4)G(5)A(6)A(7)T(8)T(9)C(10)G(11)C(12)G)(2)(3'), and its carbocyclic-nucleotide analogues in which the pentose sugar in 2'-dA residue is replaced with its carbocyclic counterpart (i.e. 2'-deoxyaristeromycin). Based on the NMR evidences, it has been shown, that 2'-deoxyaristeromycin analog exists in a dynamic equilibrium between the two forms of duplexes, one with W-C bp and the second with Hoogsteen bp in ca 1:1 ratio at lower temperature (below 35 degrees C) and as hairpin at higher temperature (from similar to 40 degrees - 60 degrees C).
UR - https://res.slu.se/id/publ/83878
U2 - 10.1080/07328319908044793
DO - 10.1080/07328319908044793
M3 - Journal article
C2 - 10474237
SN - 0732-8311
VL - 18
SP - 1593
EP - 1596
JO - Nucleosides and Nucleotides
JF - Nucleosides and Nucleotides
IS - 6-7
ER -