A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

Hongyan Xia; Lihong Liu; Ann Nordengrahn; Istvan Kiss; Malik Merza; Ronnie Eriksson; Jonas Blomberg; Sándor Belák

doi:10.1016/j.jviromet.2010.04.009

A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

Hongyan Xia
, Lihong Liu
, Ann Nordengrahn
, Istvan Kiss
, Malik Merza
, Ronnie Eriksson
, Jonas Blomberg
, Sándor Belák

Publikation: Bidrag till tidskrift › Artikel i vetenskaplig tidskrift › Peer review

Sammanfattning

This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.

Originalspråk	Engelska
Sidor (från-till)	18-21
Antal sidor	4
Tidskrift	Journal of Virological Methods
Volym	168
Nummer	1-2
DOI	https://doi.org/10.1016/j.jviromet.2010.04.009
Status	Publicerad - 2010

Nyckelord

Luminex
Microsphere immunoassay
BVDV

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10.1016/j.jviromet.2010.04.009

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@article{ece39113ad2f4403b48604d55eb04006,

title = "A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies",

abstract = "This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9\% and less than 7\%, respectively, and variability of bead conjugations is less than 6.6\%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3\%, the assay has a sensitivity of 99.4\% and a specificity of 98.3\% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.",

keywords = "Luminex, Microsphere immunoassay, BVDV, Luminex, Microsphere immunoassay, BVDV",

author = "Hongyan Xia and Lihong Liu and Ann Nordengrahn and Istvan Kiss and Malik Merza and Ronnie Eriksson and Jonas Blomberg and S{\'a}ndor Bel{\'a}k",

year = "2010",

doi = "10.1016/j.jviromet.2010.04.009",

language = "English",

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pages = "18--21",

journal = "Journal of Virological Methods",

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TY - JOUR

T1 - A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies

AU - Xia, Hongyan

AU - Liu, Lihong

AU - Nordengrahn, Ann

AU - Kiss, Istvan

AU - Merza, Malik

AU - Eriksson, Ronnie

AU - Blomberg, Jonas

AU - Belák, Sándor

PY - 2010

Y1 - 2010

N2 - This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.

AB - This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.

KW - Luminex

KW - Microsphere immunoassay

KW - BVDV

KW - Luminex

KW - Microsphere immunoassay

KW - BVDV

UR - https://res.slu.se/id/publ/60460

U2 - 10.1016/j.jviromet.2010.04.009

DO - 10.1016/j.jviromet.2010.04.009

M3 - Journal article

C2 - 20403384

SN - 0166-0934

VL - 168

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EP - 21

JO - Journal of Virological Methods

JF - Journal of Virological Methods

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