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Using a Reverse Genetics Approach to Investigate Small-Molecule Activity

Publication: Chapter in Book/Report/Conference proceedingBook chapterResearch

Abstract

Chemical genomics is a highly effective approach for understanding complex and dynamic biologicalprocesses in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a hugeabundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here wepresent an approach to characterize a chemical of interest, as well as examples of studies demonstrating aneffective combination of chemical genomics with reverse genetics strategies, drawn from recent literatureon phytohormone signalling and auxin transport.
Original languageEnglish
Title of host publicationPlant chemical genomics : methods and protocols
PublisherSpringer
Pages51-62
Number of pages12
ISBN (Electronic)978-1-62703-592-7
ISBN (Print)978-1-62703-591-0
DOIs
Publication statusPublished - 2014

Publication series

SeriesMethods in Molecular Biology
Number1056
Volume1056
ISSN1064-3745

Keywords

  • Arabidopsis mutants
  • Auxin transport
  • Chemical genomics
  • Phytohormone signalling
  • Reverse genetics

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