Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Anti Vasemägi; Jan Nilsson; Craig R Primmer

doi:10.1111/j.1471-8286.2005.00902.x

Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Anti Vasemägi
, Jan Nilsson
, Craig R Primmer

Publication: Contribution to journal › Journal article › peer-review

Abstract

An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).

Original language	English
Pages (from-to)	282-288
Number of pages	7
Journal	Molecular Ecology Notes
Volume	5
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2005.00902.x
Publication status	Published - 2005

Keywords

cDNA
EST
expressed sequence tag
microsatellite
salmonidae
Salmo salar

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10.1111/j.1471-8286.2005.00902.x

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title = "Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species",

abstract = "An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).",

keywords = "cDNA, EST, expressed sequence tag, microsatellite, salmonidae, Salmo salar, cDNA, EST, expressed sequence tag, microsatellite, salmonidae, Salmo salar",

author = "Anti Vasem{\"a}gi and Jan Nilsson and Primmer, \{Craig R\}",

year = "2005",

doi = "10.1111/j.1471-8286.2005.00902.x",

language = "English",

volume = "5",

pages = "282--288",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

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TY - JOUR

T1 - Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

AU - Vasemägi, Anti

AU - Nilsson, Jan

AU - Primmer, Craig R

PY - 2005

Y1 - 2005

N2 - An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).

AB - An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species).

KW - cDNA

KW - EST

KW - expressed sequence tag

KW - microsatellite

KW - salmonidae

KW - Salmo salar

KW - cDNA

KW - EST

KW - expressed sequence tag

KW - microsatellite

KW - salmonidae

KW - Salmo salar

UR - https://res.slu.se/id/publ/4463

U2 - 10.1111/j.1471-8286.2005.00902.x

DO - 10.1111/j.1471-8286.2005.00902.x

M3 - Journal article

SN - 1471-8278

VL - 5

SP - 282

EP - 288

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Abstract

Keywords

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