Serum tryptophan and its metabolites in female dogs undergoing ovariohysterectomy as treatment of pyometra or as elective spay surgery

This study compares serum concentrations of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), and indoleamine 2,3-dioxygenase (IDO) activity in healthy bitches and bitches with bacterial uterine infection (pyometra). The effects of surgery were also assessed by measuring these variables in both groups of dogs before and after ovariohysterectomy. Presurgery, mean (+/- standard deviation) TRP, KYN, and KYNA concentrations and IDO activity were 68.44 +/- 1.77, 2.00 +/- 0.33, 112.11 +/- 111.91 mu mol/L, and 29.22 +/- 10.10, respectively, in the healthy dogs; and 40.16 +/- 12.11, 8.27 +/- 3.94, 411.11 +/- 199.60 mu mol/L, and 205.92 +/- 154.20, respectively, in the dogs with pyometra. Tryptophan and KYN levels had normalized on suture removal (10 days after surgery) though IDO activity and KYNA concentrations remained elevated during the postoperative period compared with presurgery values in both study groups. Our results suggest that KYNA concentrations and IDO activity could be useful indicators of the inflammation induced by pyometra and could be also used to monitor recovery following ovariohysterectomy in both healthy dogs and dogs with pyometra. (C) 2015 Elsevier Inc. All rights reserved.