TY - JOUR
T1 - RAPD-PCR and PFGE as tools in the investigation of an outbreak of beta-haemolytic Streptococcus group A in a Swedish hospital
AU - Svenson, Stefan
AU - Gonzalez Rey, Carlos
AU - Krovacek, Karel
AU - Belin, A-M
AU - Henriques, B
AU - Jörbeck, H
AU - Källenius, G
AU - Norman, M
PY - 2003
Y1 - 2003
N2 - We evaluated the random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques for studying an outbreak of beta-haemolytic streptococci group A (GAS) occurred at two maternity wards at Danderyd hospital, Stockholm, Sweden. All the isolates were of T-type 8,25. The RAPD technique revealed that all RAPD-PCR profiles were identical. PFGE showed that all the patterns but one were identical. These patterns were compared with 10 different T-type GAS from the strain collection of the Swedish Institute for Infectious Disease Control (SMI) and T-type 8,25 from different years and locations. The SMI strains exhibited patterns different from each other and all different from the isolates from Danderyd hospital. Moreover, RAPD could not differentiate among the T-type 8,25 isolates from different years and locations but PFGE showed differences among the amplicons. Our results indicated that the RAPD and PFGE techniques could be efficient tools in epidemiological studies of GAS. (C) 2002 Elsevier Science Ltd. All rights reserved.
AB - We evaluated the random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques for studying an outbreak of beta-haemolytic streptococci group A (GAS) occurred at two maternity wards at Danderyd hospital, Stockholm, Sweden. All the isolates were of T-type 8,25. The RAPD technique revealed that all RAPD-PCR profiles were identical. PFGE showed that all the patterns but one were identical. These patterns were compared with 10 different T-type GAS from the strain collection of the Swedish Institute for Infectious Disease Control (SMI) and T-type 8,25 from different years and locations. The SMI strains exhibited patterns different from each other and all different from the isolates from Danderyd hospital. Moreover, RAPD could not differentiate among the T-type 8,25 isolates from different years and locations but PFGE showed differences among the amplicons. Our results indicated that the RAPD and PFGE techniques could be efficient tools in epidemiological studies of GAS. (C) 2002 Elsevier Science Ltd. All rights reserved.
KW - beta-haemolytic streptococci group A
KW - nosocomial infection
KW - T-type 8,25
KW - random amplified polymorphic DNA
KW - pulsed-field gel electrophoresis
KW - fingerprinting
KW - beta-haemolytic streptococci group A
KW - nosocomial infection
KW - T-type 8,25
KW - random amplified polymorphic DNA
KW - pulsed-field gel electrophoresis
KW - fingerprinting
UR - https://res.slu.se/id/publ/1266
U2 - 10.1016/S0147-9571(02)00019-X
DO - 10.1016/S0147-9571(02)00019-X
M3 - Journal article
C2 - 12602684
SN - 0147-9571
VL - 26
SP - 25
EP - 35
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
IS - 1
ER -