PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants

Anders Carlsson; Sten Stymne; Pierette Bouciwe-Navè; Anna Szilagyi; Alexandre Noiriel; Antoni Banas; Bangquan Huang; Hans-Erik Åkerlund; Hubert Schaller

PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants

Anders Carlsson
, Sten Stymne
, Pierette Bouciwe-Navè
, Anna Szilagyi
, Alexandre Noiriel
, Antoni Banas
, Bangquan Huang
, Hans-Erik Åkerlund
, Hubert Schaller

Publication: Contribution to conference › Conference abstract (not in scientific journal)

Abstract

The first plant gene (At1g04010) encoding a sterol ester synthesising enzyme, the phospholipid:sterol acyltransferase (PSAT) has been identified in Arabidopsis and shown to belong to the LCAT/PDAT family (1). It is the first identified intracellular enzyme catalyzing the synthesis of sterol esters by an acyl CoA-independent reaction in which acyl groups are transacylated from phospholipids to sterols. The enzyme does not utilize neutral lipids, has highest activity with phosphatidylethanolamine, a five-fold preference for the sn-2 position and utilize both saturated and unsaturated fatty acids. The results presented implicate a role for AtPSAT in regulating both the amount and the quality of the free sterols in the membrane. Changes in fluorescence and pigment composition as well as the development of a chilling phenotype in the AtPSAT T-DNA knock after a switch to lower temperatures implies an importance of AtPSAT in temperature acclimation of plants

Original language	English
Publication status	Published - 2006
Event	17th International symposium in Plant lipids - Michigan State University, USA Duration: 1 Jan 2006 → …

Conference

Conference	17th International symposium in Plant lipids
City	Michigan State University, USA
Period	2006-01-01 → …

Cite this

@conference{4759eb6b51ff4099bc3d5f65ea4b268c,

title = "PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants",

abstract = "The first plant gene (At1g04010) encoding a sterol ester synthesising enzyme, the phospholipid:sterol acyltransferase (PSAT) has been identified in Arabidopsis and shown to belong to the LCAT/PDAT family (1). It is the first identified intracellular enzyme catalyzing the synthesis of sterol esters by an acyl CoA-independent reaction in which acyl groups are transacylated from phospholipids to sterols. The enzyme does not utilize neutral lipids, has highest activity with phosphatidylethanolamine, a five-fold preference for the sn-2 position and utilize both saturated and unsaturated fatty acids. The results presented implicate a role for AtPSAT in regulating both the amount and the quality of the free sterols in the membrane. Changes in fluorescence and pigment composition as well as the development of a chilling phenotype in the AtPSAT T-DNA knock after a switch to lower temperatures implies an importance of AtPSAT in temperature acclimation of plants",

author = "Anders Carlsson and Sten Stymne and Pierette Bouciwe-Nav{\`e} and Anna Szilagyi and Alexandre Noiriel and Antoni Banas and Bangquan Huang and Hans-Erik {\AA}kerlund and Hubert Schaller",

year = "2006",

language = "English",

note = "17th International symposium in Plant lipids ; Conference date: 01-01-2006",

}

PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants. / Carlsson, Anders ; Stymne, Sten; Bouciwe-Navè, Pierette et al.
2006. Abstract from 17th International symposium in Plant lipids, Michigan State University, USA.

Publication: Contribution to conference › Conference abstract (not in scientific journal)

TY - CONF

T1 - PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants

AU - Carlsson, Anders

AU - Stymne, Sten

AU - Bouciwe-Navè, Pierette

AU - Szilagyi, Anna

AU - Noiriel, Alexandre

AU - Banas, Antoni

AU - Huang, Bangquan

AU - Åkerlund, Hans-Erik

AU - Schaller, Hubert

PY - 2006

Y1 - 2006

N2 - The first plant gene (At1g04010) encoding a sterol ester synthesising enzyme, the phospholipid:sterol acyltransferase (PSAT) has been identified in Arabidopsis and shown to belong to the LCAT/PDAT family (1). It is the first identified intracellular enzyme catalyzing the synthesis of sterol esters by an acyl CoA-independent reaction in which acyl groups are transacylated from phospholipids to sterols. The enzyme does not utilize neutral lipids, has highest activity with phosphatidylethanolamine, a five-fold preference for the sn-2 position and utilize both saturated and unsaturated fatty acids. The results presented implicate a role for AtPSAT in regulating both the amount and the quality of the free sterols in the membrane. Changes in fluorescence and pigment composition as well as the development of a chilling phenotype in the AtPSAT T-DNA knock after a switch to lower temperatures implies an importance of AtPSAT in temperature acclimation of plants

AB - The first plant gene (At1g04010) encoding a sterol ester synthesising enzyme, the phospholipid:sterol acyltransferase (PSAT) has been identified in Arabidopsis and shown to belong to the LCAT/PDAT family (1). It is the first identified intracellular enzyme catalyzing the synthesis of sterol esters by an acyl CoA-independent reaction in which acyl groups are transacylated from phospholipids to sterols. The enzyme does not utilize neutral lipids, has highest activity with phosphatidylethanolamine, a five-fold preference for the sn-2 position and utilize both saturated and unsaturated fatty acids. The results presented implicate a role for AtPSAT in regulating both the amount and the quality of the free sterols in the membrane. Changes in fluorescence and pigment composition as well as the development of a chilling phenotype in the AtPSAT T-DNA knock after a switch to lower temperatures implies an importance of AtPSAT in temperature acclimation of plants

UR - https://res.slu.se/id/publ/11146

M3 - Conference abstract (not in scientific journal)

T2 - 17th International symposium in Plant lipids

Y2 - 1 January 2006

ER -

PSAT that catalyzes an acyl CoA-independent sterol ester formation may have a role in temperature acclimation of plants

Abstract

Conference

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