Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

Tomasz Niedziela; Lennart Kenne; Czeslaw Lugowski

doi:10.1016/j.carres.2009.11.005

Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

Tomasz Niedziela
, Lennart Kenne
, Czeslaw Lugowski

Publication: Contribution to journal › Journal article › peer-review

Abstract

The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P}(n). (C) 2009 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	270-274
Number of pages	5
Journal	Carbohydrate Research
Volume	345
Issue number	2
DOIs	https://doi.org/10.1016/j.carres.2009.11.005
Publication status	Published - 2010

Keywords

Hafnia alvei
O-antigen
Lipopolysaccharide
Phosphodiester
NMR spectroscopy
MALDI-TOF mass spectrometry

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10.1016/j.carres.2009.11.005

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@article{6da8ddd36bfa471ebf625225be650f6a,

title = "Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure",

abstract = "The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: \{-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P\}(n). (C) 2009 Elsevier Ltd. All rights reserved.",

keywords = "Hafnia alvei, O-antigen, Lipopolysaccharide, Phosphodiester, NMR spectroscopy, MALDI-TOF mass spectrometry, Hafnia alvei, O-antigen, Lipopolysaccharide, Phosphodiester, NMR spectroscopy, MALDI-TOF mass spectrometry",

author = "Tomasz Niedziela and Lennart Kenne and Czeslaw Lugowski",

year = "2010",

doi = "10.1016/j.carres.2009.11.005",

language = "English",

volume = "345",

pages = "270--274",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd",

number = "2",

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TY - JOUR

T1 - Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

AU - Niedziela, Tomasz

AU - Kenne, Lennart

AU - Lugowski, Czeslaw

PY - 2010

Y1 - 2010

N2 - The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P}(n). (C) 2009 Elsevier Ltd. All rights reserved.

AB - The lipopolysaccharide (LPS) of Hafnia alvei strain PCM 1195 was obtained by the hot phenol/water method. The O-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. The structure of the O-specific polysaccharide was investigated by (1)H, (13)C, and (31)P NMR spectroscopy, MALDI-TOF MS, and GC-MS, accompanied by monosaccharide and methylation analysis. It was concluded that the O-specific polysaccharide is composed of a hexasaccharide repeating units interlinked with a phosphate group: {-> 4-alpha-D-Glcp-(1 -> 3)-alpha-L-FucpNAc-(1 -> 3)-[alpha-D-Glcp-(1 -> 4)]-alpha-D-GlcpNAc-(1 -> 3)-alpha-L-FucpNAc-(1 -> 4)-alpha-D-Glcp-(1 -> P}(n). (C) 2009 Elsevier Ltd. All rights reserved.

KW - Hafnia alvei

KW - O-antigen

KW - Lipopolysaccharide

KW - Phosphodiester

KW - NMR spectroscopy

KW - MALDI-TOF mass spectrometry

KW - Hafnia alvei

KW - O-antigen

KW - Lipopolysaccharide

KW - Phosphodiester

KW - NMR spectroscopy

KW - MALDI-TOF mass spectrometry

UR - https://res.slu.se/id/publ/59778

U2 - 10.1016/j.carres.2009.11.005

DO - 10.1016/j.carres.2009.11.005

M3 - Journal article

C2 - 20003963

SN - 0008-6215

VL - 345

SP - 270

EP - 274

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 2

ER -

Novel O-antigen of Hafnia alvei PCM 1195 lipopolysaccharide with a teichoic acid-like structure

Abstract

Keywords

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