TY - JOUR
T1 - Molecular typing of Cryptosporidium parvum associated with a diarrhoea outbreak identifies two sources of exposure
AU - Mattsson, Jens
AU - Insulander, Mona
AU - Lebbad, Marianne
AU - Björkman, Camilla
AU - Svenungsson, Elisabet
PY - 2008
Y1 - 2008
N2 - An outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800-1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive for Cryptosporidium oocysts. RFLP and sequencing analyses showed that all individuals were infected with Cryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting of C. parvum isolates are valuable in the epidemiological investigation of outbreaks.
AB - An outbreak of cryptosporidiosis associated with exposure to outdoor swimming-pool water affected an estimated 800-1000 individuals. PCR products were obtained from faecal specimens from 30 individuals who tested positive for Cryptosporidium oocysts. RFLP and sequencing analyses showed that all individuals were infected with Cryptosporidium parvum. Among the infected individuals, five had just swum in an adjacent indoor pool during the same period, and had no identified contact with individuals linked to the outdoor pool. With the use of subgenotyping based on analysis of three mini- and microsatellite loci, MS1, TP14, and GP15, we could identify two sources of exposure. One subtype was associated with the outdoor pool and another with the indoor pool. These data demonstrate that the use of mini- and microsatellite loci as markers for molecular fingerprinting of C. parvum isolates are valuable in the epidemiological investigation of outbreaks.
UR - https://res.slu.se/id/publ/18861
U2 - 10.1017/S0950268807009673
DO - 10.1017/S0950268807009673
M3 - Journal article
C2 - 17961283
SN - 0950-2688
VL - 136
SP - 1147
EP - 1152
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 8
ER -