Microdistribution of xyloglucan in differentiating Poplar cells

David Sandquist; Lada Filonova; Laura von Schantz; Mats Ohlin; Geoffrey Daniel

doi:10.15376/biores.5.2.796-807

Microdistribution of xyloglucan in differentiating Poplar cells

David Sandquist
, Lada Filonova
, Laura von Schantz
, Mats Ohlin
, Geoffrey Daniel

Publication: Contribution to journal › Journal article › peer-review

Abstract

Recent studies on the ultrastructure and composition of the gelatinous layer (g-layer) in poplar have reported findings of xyloglucan. Using correlated fluorescence, scanning- and transmission electron microscopy, we found evidence for xyloglucan present in and surrounding the g-layer, using the fucosylated xyloglucan specific CCRC-M1 antibody and the carbohydrate binding module FXG-14b. However, labeling of isolated gelatinous layer remained negative.

Original language	English
Pages (from-to)	796-807
Number of pages	12
Journal	BioResources
Volume	5
Issue number	2
DOIs	https://doi.org/10.15376/biores.5.2.796-807
Publication status	Published - 2010

Keywords

Populus tremula x tremuloides
Tension Wood
Xyloglucan
CBM
Immunogold labeling

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10.15376/biores.5.2.796-807

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title = "Microdistribution of xyloglucan in differentiating Poplar cells",

abstract = "Recent studies on the ultrastructure and composition of the gelatinous layer (g-layer) in poplar have reported findings of xyloglucan. Using correlated fluorescence, scanning- and transmission electron microscopy, we found evidence for xyloglucan present in and surrounding the g-layer, using the fucosylated xyloglucan specific CCRC-M1 antibody and the carbohydrate binding module FXG-14b. However, labeling of isolated gelatinous layer remained negative.",

keywords = "Populus tremula x tremuloides, Tension Wood, Xyloglucan, CBM, Immunogold labeling, Populus tremula x tremuloides, Tension Wood, Xyloglucan, CBM, Immunogold labeling",

author = "David Sandquist and Lada Filonova and \{von Schantz\}, Laura and Mats Ohlin and Geoffrey Daniel",

year = "2010",

doi = "10.15376/biores.5.2.796-807",

language = "English",

volume = "5",

pages = "796--807",

journal = "BioResources",

issn = "1930-2126",

publisher = "North Carolina State University",

number = "2",

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TY - JOUR

T1 - Microdistribution of xyloglucan in differentiating Poplar cells

AU - Sandquist, David

AU - Filonova, Lada

AU - von Schantz, Laura

AU - Ohlin, Mats

AU - Daniel, Geoffrey

PY - 2010

Y1 - 2010

N2 - Recent studies on the ultrastructure and composition of the gelatinous layer (g-layer) in poplar have reported findings of xyloglucan. Using correlated fluorescence, scanning- and transmission electron microscopy, we found evidence for xyloglucan present in and surrounding the g-layer, using the fucosylated xyloglucan specific CCRC-M1 antibody and the carbohydrate binding module FXG-14b. However, labeling of isolated gelatinous layer remained negative.

AB - Recent studies on the ultrastructure and composition of the gelatinous layer (g-layer) in poplar have reported findings of xyloglucan. Using correlated fluorescence, scanning- and transmission electron microscopy, we found evidence for xyloglucan present in and surrounding the g-layer, using the fucosylated xyloglucan specific CCRC-M1 antibody and the carbohydrate binding module FXG-14b. However, labeling of isolated gelatinous layer remained negative.

KW - Populus tremula x tremuloides

KW - Tension Wood

KW - Xyloglucan

KW - CBM

KW - Immunogold labeling

KW - Populus tremula x tremuloides

KW - Tension Wood

KW - Xyloglucan

KW - CBM

KW - Immunogold labeling

UR - https://res.slu.se/id/publ/60588

UR - http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_05_2_0796_Sandquist_FVOD_Microdistribution_Xyloglucan_Poplar/560

U2 - 10.15376/biores.5.2.796-807

DO - 10.15376/biores.5.2.796-807

M3 - Journal article

SN - 1930-2126

VL - 5

SP - 796

EP - 807

JO - BioResources

JF - BioResources

IS - 2

ER -

Microdistribution of xyloglucan in differentiating Poplar cells

Abstract

Keywords

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