TY - JOUR
T1 - Microarray-based detection of virulence genes in verotoxigenic Escherichia coli O157:H7 strains from Swedish cattle
AU - Söderlund, R.
AU - Aspán, Anna
AU - La Ragione, R.M.
AU - Eriksson, Erik
AU - Boqvist, Sofia
PY - 2011
Y1 - 2011
N2 - Verotoxigenic Escherichia coli (VTEC) serotype O157:H7 strains from a Swedish cattle prevalence study (n=32), and livestock-derived strains linked to human disease (n=13), were characterized by microarray and PCR detection of virulence genes. The overall aim of the study was to investigate the distribution of known virulence determinants and determine which genes are linked to increased pathogenicity in humans. A core set of 18 genes or gene variants were found in all strains, while seven genes were variably present. This suggests that the majority of VTEC O157:H7 found in Swedish cattle carry a broad repertoire of virulence genes and should be considered potentially harmful to humans. A single virulence gene type was significantly associated with strains linked to human disease cases (P=0.012), but no genetic trait to explain the increased virulence of this genotype could be found.
AB - Verotoxigenic Escherichia coli (VTEC) serotype O157:H7 strains from a Swedish cattle prevalence study (n=32), and livestock-derived strains linked to human disease (n=13), were characterized by microarray and PCR detection of virulence genes. The overall aim of the study was to investigate the distribution of known virulence determinants and determine which genes are linked to increased pathogenicity in humans. A core set of 18 genes or gene variants were found in all strains, while seven genes were variably present. This suggests that the majority of VTEC O157:H7 found in Swedish cattle carry a broad repertoire of virulence genes and should be considered potentially harmful to humans. A single virulence gene type was significantly associated with strains linked to human disease cases (P=0.012), but no genetic trait to explain the increased virulence of this genotype could be found.
KW - Molecular biology
KW - molecular epidemiology
KW - Shiga-like toxin-producing E. coli
KW - zoonotic foodborne diseases
KW - Molecular biology
KW - molecular epidemiology
KW - Shiga-like toxin-producing E. coli
KW - zoonotic foodborne diseases
UR - https://res.slu.se/id/publ/46583
U2 - 10.1017/S095026881000213X
DO - 10.1017/S095026881000213X
M3 - Journal article
C2 - 20843386
SN - 0950-2688
VL - 139
SP - 1088
EP - 1096
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 7
ER -