TY - JOUR
T1 - Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
AU - Galindo, Federico Gómez
AU - Dejmek, Petr
AU - Lundgren, Krister
AU - Rasmusson, Allan G.
AU - Vicente, António
AU - Moritz, Thomas
PY - 2009
Y1 - 2009
N2 - Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
AB - Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
UR - https://res.slu.se/id/publ/26208
U2 - 10.1007/s00425-009-0950-2
DO - 10.1007/s00425-009-0950-2
M3 - Journal article
C2 - 19495789
SN - 0032-0935
VL - 230
SP - 469
EP - 479
JO - Planta
JF - Planta
IS - 3
ER -