LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1 beta Release

Anders Lundequist; Joshua A Boyce

doi:10.1371/journal.pone.0018192

LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1 beta Release

Anders Lundequist
, Joshua A Boyce

Publication: Contribution to journal › Journal article › peer-review

Abstract

Background Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release

Original language	English
Number of pages	6
Journal	PLOS ONE
Volume	6
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0018192
Publication status	Published - 2011

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title = "LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1 beta Release",

abstract = "Background Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release",

author = "Anders Lundequist and Boyce, \{Joshua A\}",

year = "2011",

doi = "10.1371/journal.pone.0018192",

language = "English",

volume = "6",

journal = "PLOS ONE",

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number = "3",

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T1 - LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1 beta Release

AU - Lundequist, Anders

AU - Boyce, Joshua A

PY - 2011

Y1 - 2011

N2 - Background Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release

AB - Background Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. Principal Findings Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. Conclusions LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release

UR - https://res.slu.se/id/publ/58898

U2 - 10.1371/journal.pone.0018192

DO - 10.1371/journal.pone.0018192

M3 - Journal article

C2 - 21464938

SN - 1932-6203

VL - 6

JO - PLOS ONE

JF - PLOS ONE

IS - 3

ER -

LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1 beta Release

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