TY - JOUR
T1 - Localization of deformed wing virus infection in queen and drone Apis mellifera L
AU - Rodrigues De Miranda, Joachim
AU - Gauthier, Laurent
AU - Cousserans, Francois
AU - Fievet, J
AU - Tentcheva, Diana
AU - Colin, ME
AU - Bergoin, Max
PY - 2006
Y1 - 2006
N2 - The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VPI structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles.
AB - The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VPI structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles.
UR - https://res.slu.se/id/publ/51591
U2 - 10.1186/1743-422X-3-16
DO - 10.1186/1743-422X-3-16
M3 - Journal article
C2 - 16569216
SN - 1743-422X
VL - 3
JO - Virology Journal
JF - Virology Journal
ER -