Abstract
In the last decade, the accumulation and roles of small RNAs have been slowly uncovered. Recently, the RNA silencing pathways active in the male gametophyte of plants have started to be analyzed in depth. Although several studies have shed light on the small RNA populations present in the pollen, we still lack a clear picture of their regulatory activity. This chapter outlines an extraction method of mature pollen grains and its different downstream applications for the purification and detection of small RNAs.
| Original language | English |
|---|---|
| Title of host publication | Plant Germline Development |
| Publisher | Humana Press |
| Pages | 237-250 |
| Number of pages | 14 |
| ISBN (Electronic) | 978-1-4939-7286-9 |
| ISBN (Print) | 978-1-4939-7285-2 |
| DOIs | |
| Publication status | Published - 2017 |
Keywords
- Small RNAs
- RNA silencing
- Pollen grain
- Northern blot
- Transposable elements
- Sperm cells
- Arabidopsis thaliana
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