Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres

Anna Kristina Mykkänen; Seppo Hyyppä; A. Reeta Pösö; Nils Ronéus; Birgitta Essen-Gustavsson

doi:10.1016/j.rvsc.2010.03.026

Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres

Anna Kristina Mykkänen
, Seppo Hyyppä
, A. Reeta Pösö
, Nils Ronéus
, Birgitta Essen-Gustavsson

Publication: Contribution to journal › Journal article › peer-review

Abstract

Monocarboxylate transporter 1 (MCT1) and its ancillary protein CD147 facilitate efflux of lactate from the muscle. Expression of MCT-1 and CD147 were studied with immunohistochemistry in type I, IIA, IIAB and IIB fibres of equine gluteal muscle. Staining intensity of MCT1 in the cytoplasm as well as in the membranes of fibre types decreased in the order I = IIA > IIAB > IIB and correlated with the oxidative capacity. Capillaries were pronounced in the MCT1 staining. CD147 antibody stained plasma membranes of all fibre types evenly, whereas the staining in the cytoplasm followed that of MCT1. In the middle gluteal muscle the expression of MCT1 follows the oxidative capacity of muscle fibres, but the expression of CD147 in sarcolemma does not vary among fibre types. The use of horse specific MCT1 and CD147 antibodies can in future studies help to evaluate lactate efflux from different muscle fibre types. (C) 2010 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	432-437
Number of pages	6
Journal	Research in Veterinary Science
Volume	89
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2010.03.026
Publication status	Published - 2010

Keywords

Horse
Muscle
Lactate
MCT1
CD147
Immunohistochemistry

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10.1016/j.rvsc.2010.03.026

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title = "Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres",

abstract = "Monocarboxylate transporter 1 (MCT1) and its ancillary protein CD147 facilitate efflux of lactate from the muscle. Expression of MCT-1 and CD147 were studied with immunohistochemistry in type I, IIA, IIAB and IIB fibres of equine gluteal muscle. Staining intensity of MCT1 in the cytoplasm as well as in the membranes of fibre types decreased in the order I = IIA > IIAB > IIB and correlated with the oxidative capacity. Capillaries were pronounced in the MCT1 staining. CD147 antibody stained plasma membranes of all fibre types evenly, whereas the staining in the cytoplasm followed that of MCT1. In the middle gluteal muscle the expression of MCT1 follows the oxidative capacity of muscle fibres, but the expression of CD147 in sarcolemma does not vary among fibre types. The use of horse specific MCT1 and CD147 antibodies can in future studies help to evaluate lactate efflux from different muscle fibre types. (C) 2010 Elsevier Ltd. All rights reserved.",

keywords = "Horse, Muscle, Lactate, MCT1, CD147, Immunohistochemistry, Horse, Muscle, Lactate, MCT1, CD147, Immunohistochemistry",

author = "Mykk{\"a}nen, \{Anna Kristina\} and Seppo Hyypp{\"a} and P{\"o}s{\"o}, \{A. Reeta\} and Nils Ron{\'e}us and Birgitta Essen-Gustavsson",

year = "2010",

doi = "10.1016/j.rvsc.2010.03.026",

language = "English",

volume = "89",

pages = "432--437",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres

AU - Mykkänen, Anna Kristina

AU - Hyyppä, Seppo

AU - Pösö, A. Reeta

AU - Ronéus, Nils

AU - Essen-Gustavsson, Birgitta

PY - 2010

Y1 - 2010

N2 - Monocarboxylate transporter 1 (MCT1) and its ancillary protein CD147 facilitate efflux of lactate from the muscle. Expression of MCT-1 and CD147 were studied with immunohistochemistry in type I, IIA, IIAB and IIB fibres of equine gluteal muscle. Staining intensity of MCT1 in the cytoplasm as well as in the membranes of fibre types decreased in the order I = IIA > IIAB > IIB and correlated with the oxidative capacity. Capillaries were pronounced in the MCT1 staining. CD147 antibody stained plasma membranes of all fibre types evenly, whereas the staining in the cytoplasm followed that of MCT1. In the middle gluteal muscle the expression of MCT1 follows the oxidative capacity of muscle fibres, but the expression of CD147 in sarcolemma does not vary among fibre types. The use of horse specific MCT1 and CD147 antibodies can in future studies help to evaluate lactate efflux from different muscle fibre types. (C) 2010 Elsevier Ltd. All rights reserved.

AB - Monocarboxylate transporter 1 (MCT1) and its ancillary protein CD147 facilitate efflux of lactate from the muscle. Expression of MCT-1 and CD147 were studied with immunohistochemistry in type I, IIA, IIAB and IIB fibres of equine gluteal muscle. Staining intensity of MCT1 in the cytoplasm as well as in the membranes of fibre types decreased in the order I = IIA > IIAB > IIB and correlated with the oxidative capacity. Capillaries were pronounced in the MCT1 staining. CD147 antibody stained plasma membranes of all fibre types evenly, whereas the staining in the cytoplasm followed that of MCT1. In the middle gluteal muscle the expression of MCT1 follows the oxidative capacity of muscle fibres, but the expression of CD147 in sarcolemma does not vary among fibre types. The use of horse specific MCT1 and CD147 antibodies can in future studies help to evaluate lactate efflux from different muscle fibre types. (C) 2010 Elsevier Ltd. All rights reserved.

KW - Horse

KW - Muscle

KW - Lactate

KW - MCT1

KW - CD147

KW - Immunohistochemistry

KW - Horse

KW - Muscle

KW - Lactate

KW - MCT1

KW - CD147

KW - Immunohistochemistry

UR - https://res.slu.se/id/publ/60990

U2 - 10.1016/j.rvsc.2010.03.026

DO - 10.1016/j.rvsc.2010.03.026

M3 - Journal article

C2 - 20435326

SN - 0034-5288

VL - 89

SP - 432

EP - 437

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

Immunohistochemical analysis of MCT1 and CD147 in equine skeletal muscle fibres

Abstract

Keywords

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