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Identification and verification of microRNAs by high-throughput sequencing

  • J. Hällman
  • , L. Avesson
  • , J. Reimegård
  • , M. Käller
  • , F. Söderbom

    Publication: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs. © Springer Science+Business Media, LLC 2013.
    Original languageEnglish
    Title of host publicationDictyostelium discoideum Protocols
    PublisherHumana Press Inc.
    Pages125-138
    Number of pages14
    ISBN (Electronic)978-1-62703-302-2
    ISBN (Print)9781627033015
    DOIs
    Publication statusPublished - 2013

    Publication series

    SeriesMethods in Molecular Biology
    Number983
    Volume983
    ISSN1064-3745

    Keywords

    • Bioinformatics
    • High-throughput sequencing
    • MicroRNA
    • Northern blot
    • Small RNA

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