Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis

Thomas W. Jeffries; Igor V. Grigoriev; Jane Grimwood; Jose M. Laplaza; Andrea Aerts; Asaf Salamov; Jeremy Schmutz; Erika Lindquist; Paramvir Dehal; Harris Shapiro; Yong-Su Jin; Volkmar Passoth; Paul M. Richardson

doi:10.1038/nbt1290

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis

Thomas W. Jeffries
, Igor V. Grigoriev
, Jane Grimwood
, Jose M. Laplaza
, Andrea Aerts
, Asaf Salamov
, Jeremy Schmutz
, Erika Lindquist
, Paramvir Dehal
, Harris Shapiro
, Yong-Su Jin
, Volkmar Passoth
, Paul M. Richardson

Publication: Contribution to journal › Journal article › peer-review

Abstract

Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.

Original language	English
Pages (from-to)	319-326
Number of pages	8
Journal	Nature Biotechnology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1038/nbt1290
Publication status	Published - 2007

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title = "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis",

abstract = "Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.",

author = "Jeffries, \{Thomas W.\} and Grigoriev, \{Igor V.\} and Jane Grimwood and Laplaza, \{Jose M.\} and Andrea Aerts and Asaf Salamov and Jeremy Schmutz and Erika Lindquist and Paramvir Dehal and Harris Shapiro and Yong-Su Jin and Volkmar Passoth and Richardson, \{Paul M.\}",

year = "2007",

doi = "10.1038/nbt1290",

language = "English",

volume = "25",

pages = "319--326",

journal = "Nature Biotechnology",

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T1 - Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis

AU - Jeffries, Thomas W.

AU - Grigoriev, Igor V.

AU - Grimwood, Jane

AU - Laplaza, Jose M.

AU - Aerts, Andrea

AU - Salamov, Asaf

AU - Schmutz, Jeremy

AU - Lindquist, Erika

AU - Dehal, Paramvir

AU - Shapiro, Harris

AU - Jin, Yong-Su

AU - Passoth, Volkmar

AU - Richardson, Paul M.

PY - 2007

Y1 - 2007

N2 - Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.

AB - Xylose is a major constituent of plant lignocellulose, and its fermentation is important for the bioconversion of plant biomass to fuels and chemicals. Pichia stipitis is a well-studied, native xylose-fermenting yeast. The mechanism and regulation of xylose metabolism in P. stipitis have been characterized and genes from P. stipitis have been used to engineer xylose metabolism in Saccharomyces cerevisiae. We have sequenced and assembled the complete genome of P. stipitis. The sequence data have revealed unusual aspects of genome organization, numerous genes for bioconversion, a preliminary insight into regulation of central metabolic pathways and several examples of colocalized genes with related functions. The genome sequence provides insight into how P. stipitis regulates its redox balance while very efficiently fermenting xylose under microaerobic conditions.

UR - https://res.slu.se/id/publ/13637

U2 - 10.1038/nbt1290

DO - 10.1038/nbt1290

M3 - Journal article

C2 - 17334359

SN - 1087-0156

VL - 25

SP - 319

EP - 326

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 3

ER -

Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis

Abstract

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