Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

Jianwei Jiang; Ke Zhang; Sheng Cheng; Qianwen Nie; Shen-xian Zhou; Qingqing Chen; Jinglong Zhou; Xiao Zhen; Xue Ting Li; Tong Wen Zhen; Mingyue Xu; Tom Hsiang; Zhengxiang Sun; Yi Zhou

doi:10.1007/s00572-019-00904-3

Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

Jianwei Jiang
, Ke Zhang
, Sheng Cheng
, Qianwen Nie
, Shen-xian Zhou
, Qingqing Chen
, Jinglong Zhou
, Xiao Zhen
, Xue Ting Li
, Tong Wen Zhen
, Mingyue Xu
, Tom Hsiang
, Zhengxiang Sun
, Yi Zhou

Department of Forest Mycology and Plant Pathology

Publication: Contribution to journal › Journal article › peer-review

Abstract

Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.

Original language	English
Pages (from-to)	531-540
Number of pages	10
Journal	Mycorrhiza
Volume	29
Issue number	5
DOIs	https://doi.org/10.1007/s00572-019-00904-3
Publication status	Published - 2019

Keywords

Mycorrhizal fungus
Fusarium oxysporum
GFP transformation
Hyphal peloton

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10.1007/s00572-019-00904-3

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title = "Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus",

abstract = "Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.",

keywords = "Mycorrhizal fungus, Fusarium oxysporum, GFP transformation, Hyphal peloton, Mycorrhizal fungus, Fusarium oxysporum, GFP transformation, Hyphal peloton",

author = "Jianwei Jiang and Ke Zhang and Sheng Cheng and Qianwen Nie and Shen-xian Zhou and Qingqing Chen and Jinglong Zhou and Xiao Zhen and Li, \{Xue Ting\} and Zhen, \{Tong Wen\} and Mingyue Xu and Tom Hsiang and Zhengxiang Sun and Yi Zhou",

year = "2019",

doi = "10.1007/s00572-019-00904-3",

language = "English",

volume = "29",

pages = "531--540",

journal = "Mycorrhiza",

issn = "0940-6360",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "5",

}

TY - JOUR

T1 - Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

AU - Jiang, Jianwei

AU - Zhang, Ke

AU - Cheng, Sheng

AU - Nie, Qianwen

AU - Zhou, Shen-xian

AU - Chen, Qingqing

AU - Zhou, Jinglong

AU - Zhen, Xiao

AU - Li, Xue Ting

AU - Zhen, Tong Wen

AU - Xu, Mingyue

AU - Hsiang, Tom

AU - Sun, Zhengxiang

AU - Zhou, Yi

PY - 2019

Y1 - 2019

N2 - Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.

AB - Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.

KW - Mycorrhizal fungus

KW - Fusarium oxysporum

KW - GFP transformation

KW - Hyphal peloton

KW - Mycorrhizal fungus

KW - Fusarium oxysporum

KW - GFP transformation

KW - Hyphal peloton

UR - https://res.slu.se/id/publ/102711

UR - https://link.springer.com/article/10.1007/s00572-019-00904-3

U2 - 10.1007/s00572-019-00904-3

DO - 10.1007/s00572-019-00904-3

M3 - Journal article

SN - 0940-6360

VL - 29

SP - 531

EP - 540

JO - Mycorrhiza

JF - Mycorrhiza

IS - 5

ER -

Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus

Abstract

Keywords

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