Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability

a Morillo Rodríguez; C Ortega Ferrusola; B Macías García; Jane Morrell; H Rodríguez Martínez; JA Tapia; FJ Peña

doi:10.1016/j.anireprosci.2011.07.009

Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability

a Morillo Rodríguez
, C Ortega Ferrusola
, B Macías García
, Jane Morrell
, H Rodríguez Martínez
, JA Tapia
, FJ Peña

Publication: Contribution to journal › Journal article › peer-review

Abstract

Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual. (C) 2011 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	78-83
Number of pages	6
Journal	Animal Reproduction Science
Volume	127
Issue number	1-2
DOIs	https://doi.org/10.1016/j.anireprosci.2011.07.009
Publication status	Published - 2011

Keywords

Stallion
Frozen sperm
Flow cytometry
CASA

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10.1016/j.anireprosci.2011.07.009

Cite this

Morillo Rodríguez, A., Ortega Ferrusola, C., Macías García, B., Morrell, J., Rodríguez Martínez, H., Tapia, JA., & Peña, FJ. (2011). Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability. Animal Reproduction Science, 127(1-2), 78-83. https://doi.org/10.1016/j.anireprosci.2011.07.009

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title = "Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability",

abstract = "Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17\%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14\%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual. (C) 2011 Elsevier B.V. All rights reserved.",

keywords = "Stallion, Frozen sperm, Flow cytometry, CASA, Stallion, Frozen sperm, Flow cytometry, CASA",

author = "\{Morillo Rodr{\'i}guez\}, a and \{Ortega Ferrusola\}, C and \{Mac{\'i}as Garc{\'i}a\}, B and Jane Morrell and \{Rodr{\'i}guez Mart{\'i}nez\}, H and JA Tapia and FJ Pe{\~n}a",

year = "2011",

doi = "10.1016/j.anireprosci.2011.07.009",

language = "English",

volume = "127",

pages = "78--83",

journal = "Animal Reproduction Science",

issn = "0378-4320",

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}

Morillo Rodríguez, A, Ortega Ferrusola, C, Macías García, B, Morrell, J, Rodríguez Martínez, H, Tapia, JA & Peña, FJ 2011, 'Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability', Animal Reproduction Science, vol. 127, no. 1-2, pp. 78-83. https://doi.org/10.1016/j.anireprosci.2011.07.009

TY - JOUR

T1 - Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability

AU - Morillo Rodríguez, a

AU - Ortega Ferrusola, C

AU - Macías García, B

AU - Morrell, Jane

AU - Rodríguez Martínez, H

AU - Tapia, JA

AU - Peña, FJ

PY - 2011

Y1 - 2011

N2 - Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual. (C) 2011 Elsevier B.V. All rights reserved.

AB - Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual. (C) 2011 Elsevier B.V. All rights reserved.

KW - Stallion

KW - Frozen sperm

KW - Flow cytometry

KW - CASA

KW - Stallion

KW - Frozen sperm

KW - Flow cytometry

KW - CASA

UR - https://res.slu.se/id/publ/46251

U2 - 10.1016/j.anireprosci.2011.07.009

DO - 10.1016/j.anireprosci.2011.07.009

M3 - Journal article

C2 - 21821371

SN - 0378-4320

VL - 127

SP - 78

EP - 83

JO - Animal Reproduction Science

JF - Animal Reproduction Science

IS - 1-2

ER -

Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability

Abstract

Keywords

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