TY - JOUR
T1 - Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
AU - Ramesh, Vetukuri
AU - Kalyandurg, Pruthvi Balachandra
AU - Saripella, Ganapathi Varma
AU - Sen, Diya
AU - Gil, José Fernando
AU - Lukhovitskaya, Nina
AU - Grenville-Briggs, Laura
AU - Savenkov, Eugene
PY - 2020
Y1 - 2020
N2 - Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5 '-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.
AB - Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5 '-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.
UR - https://res.slu.se/id/publ/108415
U2 - 10.1007/s00705-020-04832-y
DO - 10.1007/s00705-020-04832-y
M3 - Journal article
SN - 0304-8608
VL - 165
SP - 2953
EP - 2959
JO - Archives of Virology
JF - Archives of Virology
IS - 12
ER -