Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

Kiran Kumar Jagarlamudi; Liya Wang; Staffan Eriksson

doi:10.1080/15257770.2018.1478094

Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

Kiran Kumar Jagarlamudi
, Liya Wang
, Staffan Eriksson

Publication: Contribution to journal › Journal article › peer-review

Abstract

In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.

Original language	English
Pages (from-to)	679-686
Number of pages	8
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	37
Issue number	12
DOIs	https://doi.org/10.1080/15257770.2018.1478094
Publication status	Published - 2018

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Doxorubicin
TK 210 ELISA
Thymidine kinase 1

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10.1080/15257770.2018.1478094

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title = "Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development",

abstract = "In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.",

keywords = "Doxorubicin, TK 210 ELISA, Thymidine kinase 1, Doxorubicin, TK 210 ELISA, Thymidine kinase 1",

author = "Jagarlamudi, \{Kiran Kumar\} and Liya Wang and Staffan Eriksson",

year = "2018",

doi = "10.1080/15257770.2018.1478094",

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T1 - Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

AU - Jagarlamudi, Kiran Kumar

AU - Wang, Liya

AU - Eriksson, Staffan

PY - 2018

Y1 - 2018

N2 - In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.

AB - In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.

KW - Doxorubicin

KW - TK 210 ELISA

KW - Thymidine kinase 1

KW - Doxorubicin

KW - TK 210 ELISA

KW - Thymidine kinase 1

UR - https://res.slu.se/id/publ/99372

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DO - 10.1080/15257770.2018.1478094

M3 - Journal article

SN - 1525-7770

VL - 37

SP - 679

EP - 686

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 12

ER -

Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

Abstract

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Keywords

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