Cytokine induction by immunostimulatory DNA in porcine PBMC is impaired by a hairpin forming sequence motif from the genome of Porcine Circovirus type 2 (PCV2)

The effect of ODN PCV2/1 was studied further by monitoring the expression of mRNA for IFN-alpha, IL-12p40, IL-10, IL-6, IFN-gamma, IL-1 beta, TGF-beta, and TNF-alpha in cultures of poPBMC stimulated with ODN 2216 or Poly I:C. Results from qPCR analyses showed that ODN PCV2/1 clearly inhibited the expression of IFN-alpha, IL-12p40, IL-10 and IL-6 when induced by ODN 2216, but did not seem to affect any of the cytokines examined when induced by Poly I:C. Initial studies using confocal microscopy and fluorochrome labelled ODNs indicate that ODN 2216 and ODN PCV2/1 co-localize in subpopulations of poPBMC. (C) 2010 Elsevier B.V. All rights reserved.