Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

D von Blumröder; G Schares; R Norton; DJL Williams; I Esteban-Redondo; S Wright; C Björkman; J Frössling; V Risco-Castillo; A Fernández-García; LM Ortega-Mora; H Sager; A Hemphill; C van Maanen; W Wouda; FJ Conraths

doi:10.1016/j.vetpar.2003.12.010

Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

D von Blumröder
, G Schares
, R Norton
, DJL Williams
, I Esteban-Redondo
, S Wright
, C Björkman
, J Frössling
, V Risco-Castillo
, A Fernández-García
, LM Ortega-Mora
, H Sager
, A Hemphill
, C van Maanen
, W Wouda
, FJ Conraths

Publication: Contribution to journal › Journal article › peer-review

Abstract

Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	45983
Number of pages	12
Journal	Veterinary Parasitology
Volume	120
Issue number	45659
DOIs	https://doi.org/10.1016/j.vetpar.2003.12.010
Publication status	Published - 2004

Keywords

Neospora caninum
enzyme-linked immunosorbent assay
indirect fluorescent antibody test
cut-off
TG-ROC
test standardisation

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10.1016/j.vetpar.2003.12.010

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von Blumröder, D., Schares, G., Norton, R., Williams, DJL., Esteban-Redondo, I., Wright, S., Björkman, C., Frössling, J., Risco-Castillo, V., Fernández-García, A., Ortega-Mora, LM., Sager, H., Hemphill, A., van Maanen, C., Wouda, W., & Conraths, FJ. (2004). Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines. Veterinary Parasitology, 120(45659), 45983. https://doi.org/10.1016/j.vetpar.2003.12.010

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title = "Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines",

abstract = "Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.",

keywords = "Neospora caninum, enzyme-linked immunosorbent assay, indirect fluorescent antibody test, cut-off, TG-ROC, test standardisation, Neospora caninum, enzyme-linked immunosorbent assay, indirect fluorescent antibody test, cut-off, TG-ROC, test standardisation",

author = "\{von Blumr{\"o}der\}, D and G Schares and R Norton and DJL Williams and I Esteban-Redondo and S Wright and C Bj{\"o}rkman and J Fr{\"o}ssling and V Risco-Castillo and A Fern{\'a}ndez-Garc{\'i}a and LM Ortega-Mora and H Sager and A Hemphill and \{van Maanen\}, C and W Wouda and FJ Conraths",

year = "2004",

doi = "10.1016/j.vetpar.2003.12.010",

language = "English",

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pages = "45983",

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von Blumröder, D, Schares, G, Norton, R, Williams, DJL, Esteban-Redondo, I, Wright, S, Björkman, C, Frössling, J, Risco-Castillo, V, Fernández-García, A, Ortega-Mora, LM, Sager, H, Hemphill, A, van Maanen, C, Wouda, W & Conraths, FJ 2004, 'Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines', Veterinary Parasitology, vol. 120, no. 45659, pp. 45983. https://doi.org/10.1016/j.vetpar.2003.12.010

TY - JOUR

T1 - Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

AU - von Blumröder, D

AU - Schares, G

AU - Norton, R

AU - Williams, DJL

AU - Esteban-Redondo, I

AU - Wright, S

AU - Björkman, C

AU - Frössling, J

AU - Risco-Castillo, V

AU - Fernández-García, A

AU - Ortega-Mora, LM

AU - Sager, H

AU - Hemphill, A

AU - van Maanen, C

AU - Wouda, W

AU - Conraths, FJ

PY - 2004

Y1 - 2004

N2 - Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

AB - Various existing serological tests were compared with a standard panel of 523 sera in a multicentred study across Europe. Well characterised sera from animals that were experimentally or naturally infected with Neospora caninum as well as sera from cattle deemed uninfected with N. caninum were provided by the participants of the study and analysed in several commercial (CHEKIT(R) Dr. Bommeli/Intervet, CIVTESTTM BOVIS NEOSPORA Hipra, Cypress Diagnostics C.V., Herd Check((R)) IDEXX, Mastazyme(TM) MAST Diagnostics, P38-ELISA Animal Welfare and Food Safety GmbH (AFOSA)) as well as in-house assays (five ELISAs and one IFAT). Most tests showed a high level of agreement in the interpretation of the test results (positive or negative). A further distinct increase in agreement between tests was obtained after the application of standardised cut-offs offered by a two-graph receiver operating characteristic analysis. This procedure allows a standardised interpretation of results obtained with different tests used in independent, parallel seroepidemiological studies. (C) 2004 Elsevier B.V. All rights reserved.

KW - Neospora caninum

KW - enzyme-linked immunosorbent assay

KW - indirect fluorescent antibody test

KW - cut-off

KW - TG-ROC

KW - test standardisation

KW - Neospora caninum

KW - enzyme-linked immunosorbent assay

KW - indirect fluorescent antibody test

KW - cut-off

KW - TG-ROC

KW - test standardisation

UR - https://res.slu.se/id/publ/2726

U2 - 10.1016/j.vetpar.2003.12.010

DO - 10.1016/j.vetpar.2003.12.010

M3 - Journal article

C2 - 15019139

SN - 0304-4017

VL - 120

SP - 45983

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 45659

ER -

Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines

Abstract

Keywords

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