TY - JOUR
T1 - Co-cultivation of antifungal Lactobacillus plantarum MiLAB 393 and Aspergillus nidulans, evaluation of effects on fungal growth and protein expression
AU - Ström, Katrin
AU - Schnürer, Johan
AU - Melin, Petter
PY - 2005
Y1 - 2005
N2 - The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory metabolites produced by L. plantarum MiLAB 393, cyclo(L-Phe-L-Pro), lactic acid and 3-phenyllactic acid, were also investigated by addition of pure compounds to the growth medium of A. nidulans. The co-cultivation strongly affected the morphology of the fungal mycelium and decreased the biomass to 36% of control. Co-cultivation with Lactobacillus coryniformis MiLAB 123 gave only marginal morphological changes and minor biomass reduction, suggesting specific effects of L. plantarum MiLAB 393. The amount of several A. nidulans-proteins was increased during cocultivation and by all of the inhibiting substances. This study shows that the growth of A. nidulans is inhibited during co-cultivation with L. plantarum MiLAB 393 and that the expression of fungal proteins is altered. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
AB - The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory metabolites produced by L. plantarum MiLAB 393, cyclo(L-Phe-L-Pro), lactic acid and 3-phenyllactic acid, were also investigated by addition of pure compounds to the growth medium of A. nidulans. The co-cultivation strongly affected the morphology of the fungal mycelium and decreased the biomass to 36% of control. Co-cultivation with Lactobacillus coryniformis MiLAB 123 gave only marginal morphological changes and minor biomass reduction, suggesting specific effects of L. plantarum MiLAB 393. The amount of several A. nidulans-proteins was increased during cocultivation and by all of the inhibiting substances. This study shows that the growth of A. nidulans is inhibited during co-cultivation with L. plantarum MiLAB 393 and that the expression of fungal proteins is altered. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
KW - antifungal activity
KW - two dimensional gel electrophoresis
KW - 3-phenyl lactic acid
KW - cyclic dipeptide
KW - antifungal activity
KW - two dimensional gel electrophoresis
KW - 3-phenyl lactic acid
KW - cyclic dipeptide
UR - https://res.slu.se/id/publ/5789
U2 - 10.1016/j.femsle.2005.03.047
DO - 10.1016/j.femsle.2005.03.047
M3 - Journal article
C2 - 15869970
SN - 0378-1097
VL - 246
SP - 119
EP - 124
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -