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Antisense oligodeoxynucleotide inhibition as a potent diagnostic tool for gene function in plant biology

  • Chuanxin Sun
  • , Haile Ghebramedhin
  • , Anna-Stina Höglund
  • , Christer Jansson

Publication: Other publicationResearchpeer-review

Abstract

Antisense oligodeoxynucleotide (ODN) inhibition emerges as an effective means for probing gene function in plant cells. Employing this method we have established the importance of the SUSIBA2 transcription factor for regulation of starch synthesis in barley endosperm, and arrived at a model for the role of the SUSIBAs in sugar signaling and source-sink commutation during cereal endosperm development. In this addendum we provide additional data demonstrating the suitability of the antisense ODN technology in studies on starch branching enzyme activities in barley leaves. We also comment on the mechanism for ODN uptake in plant cells
Original languageEnglish
Number of pages3
Volume3
DOIs
Publication statusPublished - 2008

Publication series

SeriesPlant Signaling and Behavior
Number5
Volume3
ISSN1559-2316

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