Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods

Flemming H. Larsen; Birgitta Essen-Gustavsson; Marianne Jensen Waern; René Lametsch; Anders Karlsson; Gunilla Lindahl

doi:10.1021/jf201822p

Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods

Flemming H. Larsen
, Birgitta Essen-Gustavsson
, Marianne Jensen Waern
, René Lametsch
, Anders Karlsson
, Gunilla Lindahl

Publication: Contribution to journal › Journal article › peer-review

Abstract

Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

Original language	English
Pages (from-to)	11895-11902
Number of pages	8
Journal	Journal of Agricultural and Food Chemistry
Volume	59
Issue number	22
DOIs	https://doi.org/10.1021/jf201822p
Publication status	Published - 2011

Keywords

glycogen
H-1 nuclear magnetic resonance (H-1 NMR)
enzymatic analysis
pork
RN-gene
PRKAG3 mutation

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10.1021/jf201822p

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@article{a9eb81d0985e4ec1ad6b699d47a32504,

title = "Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods",

abstract = "Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.",

keywords = "glycogen, H-1 nuclear magnetic resonance (H-1 NMR), enzymatic analysis, pork, RN-gene, PRKAG3 mutation, glycogen, H-1 nuclear magnetic resonance (H-1 NMR), enzymatic analysis, pork, RN-gene, PRKAG3 mutation",

author = "Larsen, \{Flemming H.\} and Birgitta Essen-Gustavsson and \{Jensen Waern\}, Marianne and Ren{\'e} Lametsch and Anders Karlsson and Gunilla Lindahl",

year = "2011",

doi = "10.1021/jf201822p",

language = "English",

volume = "59",

pages = "11895--11902",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "22",

}

Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods. / Larsen, Flemming H.; Essen-Gustavsson, Birgitta; Jensen Waern, Marianne et al.
In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 22, 2011, p. 11895-11902.

Publication: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods

AU - Larsen, Flemming H.

AU - Essen-Gustavsson, Birgitta

AU - Jensen Waern, Marianne

AU - Lametsch, René

AU - Karlsson, Anders

AU - Lindahl, Gunilla

PY - 2011

Y1 - 2011

N2 - Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

AB - Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

KW - glycogen

KW - H-1 nuclear magnetic resonance (H-1 NMR)

KW - enzymatic analysis

KW - pork

KW - RN-gene

KW - PRKAG3 mutation

KW - glycogen

KW - H-1 nuclear magnetic resonance (H-1 NMR)

KW - enzymatic analysis

KW - pork

KW - RN-gene

KW - PRKAG3 mutation

UR - https://res.slu.se/id/publ/43199

UR - http://pubs.acs.org/doi/abs/10.1021/jf201822p

U2 - 10.1021/jf201822p

DO - 10.1021/jf201822p

M3 - Journal article

C2 - 22004283

SN - 0021-8561

VL - 59

SP - 11895

EP - 11902

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 22

ER -

Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods

Abstract

Keywords

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