TY - JOUR
T1 - Agrobacterium tumefaciens: From crown gall tumors to genetic transformation
AU - Păcurar, Daniel I.
AU - Thordal-Christensen, Hans
AU - Pacurar, Monica
AU - Pamfil, Doru
AU - Botez, Constantin
AU - Bellini, Catherine
PY - 2011
Y1 - 2011
N2 - The phytopathogenic bacterium Agrobacterium tumefaciens is the causative agent of crown gall disease on a broad range of plant species. For more than a century this pathogen has fascinated biologists for various reasons. It has been believed that disclosing the mystery of the crown gall tumour disease in plants might help to understand the mechanisms of oncogenesis in general, and eventually use this knowledge to cure cancer in animals and humans. However, as the performer of the only known case of trans-kingdom DNA transfer, this natural genetic engineer is recognised by nowadays biologists particularly for this feature, and Agrobacterium-mediated genetic transformation has become the preferred method to generate transgenic plants. This review briefly highlights the key steps in DNA transfer during natural A. tumefaciens infection and the subsequent development of crown gall disease. It will focus on the adaptation and use of this proof of principle for the purpose of genetic transformation. (C) 2011 Elsevier Ltd. All rights reserved.
AB - The phytopathogenic bacterium Agrobacterium tumefaciens is the causative agent of crown gall disease on a broad range of plant species. For more than a century this pathogen has fascinated biologists for various reasons. It has been believed that disclosing the mystery of the crown gall tumour disease in plants might help to understand the mechanisms of oncogenesis in general, and eventually use this knowledge to cure cancer in animals and humans. However, as the performer of the only known case of trans-kingdom DNA transfer, this natural genetic engineer is recognised by nowadays biologists particularly for this feature, and Agrobacterium-mediated genetic transformation has become the preferred method to generate transgenic plants. This review briefly highlights the key steps in DNA transfer during natural A. tumefaciens infection and the subsequent development of crown gall disease. It will focus on the adaptation and use of this proof of principle for the purpose of genetic transformation. (C) 2011 Elsevier Ltd. All rights reserved.
KW - Agrobacterium tumefaciens
KW - Crown gall
KW - Genetic transformation
KW - Agrobacterium tumefaciens
KW - Crown gall
KW - Genetic transformation
UR - https://res.slu.se/id/publ/59251
U2 - 10.1016/j.pmpp.2011.06.004
DO - 10.1016/j.pmpp.2011.06.004
M3 - Review article
SN - 0885-5765
VL - 76
SP - 76
EP - 81
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
IS - 2
ER -