TY - JOUR
T1 - A Bistable Circuit Involving SCARECROW-RETINOBLASTOMA Integrates Cues to Inform Asymmetric Stem Cell Division
AU - Cruz-Ramirez, Alfredo
AU - Diaz-Trivino, Sara
AU - Blilou, Ikram
AU - Grieneisen, Veronica A.
AU - Sozzani, Rosangela
AU - Zamioudis, Christos
AU - Miskolczi, Pal
AU - Nieuwland, Jeroen
AU - Benjamins, Rene
AU - Dhonukshe, Pankaj
AU - Caballero-Perez, Juan
AU - Horvath, Beatrix
AU - Long, Yuchen
AU - Mahonen, Ari Pekka
AU - Zhang, Hongtao
AU - Xu, Jian
AU - Murray, James A. H.
AU - Benfey, Philip N.
AU - Bako, Laszlo
AU - Maree, Athanasius F. M.
AU - Scheres, Ben
PY - 2012
Y1 - 2012
N2 - In plants, where cells cannot migrate, asymmetric cell divisions (ACDs) must be confined to the appropriate spatial context. We investigate tissue-generating asymmetric divisions in a stem cell daughter within the Arabidopsis root. Spatial restriction of these divisions requires physical binding of the stem cell regulator SCARECROW (SCR) by the RETINOBLASTOM-RELATED (RBR) protein. In the stem cell niche, SCR activity is counteracted by phosphorylation of RBR through a cyclinD6;1-CDK complex. This cyclin is itself under transcriptional control of SCR and its partner SHORT ROOT (SHR), creating a robust bistable circuit with either high or low SHR-SCR complex activity. Auxin biases this circuit by promoting CYCD6;1 transcription. Mathematical modeling shows that ACDs are only switched on after integration of radial and longitudinal information, determined by SHR and auxin distribution, respectively. Coupling of cell-cycle progression to protein degradation resets the circuit, resulting in a "flip flop" that constrains asymmetric cell division to the stem cell region.
AB - In plants, where cells cannot migrate, asymmetric cell divisions (ACDs) must be confined to the appropriate spatial context. We investigate tissue-generating asymmetric divisions in a stem cell daughter within the Arabidopsis root. Spatial restriction of these divisions requires physical binding of the stem cell regulator SCARECROW (SCR) by the RETINOBLASTOM-RELATED (RBR) protein. In the stem cell niche, SCR activity is counteracted by phosphorylation of RBR through a cyclinD6;1-CDK complex. This cyclin is itself under transcriptional control of SCR and its partner SHORT ROOT (SHR), creating a robust bistable circuit with either high or low SHR-SCR complex activity. Auxin biases this circuit by promoting CYCD6;1 transcription. Mathematical modeling shows that ACDs are only switched on after integration of radial and longitudinal information, determined by SHR and auxin distribution, respectively. Coupling of cell-cycle progression to protein degradation resets the circuit, resulting in a "flip flop" that constrains asymmetric cell division to the stem cell region.
UR - https://res.slu.se/id/publ/42654
U2 - 10.1016/j.cell.2012.07.017
DO - 10.1016/j.cell.2012.07.017
M3 - Journal article
C2 - 22921914
SN - 0092-8674
VL - 150
SP - 1002
EP - 1015
JO - Cell
JF - Cell
IS - 5
ER -